Abstract
The C_(31)-triterpene, eburicoic acid, has been biosynthesized by allowing P. sulfureus to grow on a medium containing methyl-labeled acetate. By degradation of the acid, it has been shown that the carboxyl carbon, C_(21), and the two methyl groups, C_(30) and C_(31), of the gem-dimethyl group of ring A are derived from the methyl of acetate. Such a distribution is predicted on the basis of the squalene hypothesis of a universal biosynthetic mechanism leading to both the triterpenes and the sterols. It also was found that the extra carbon atom, C_(28), found on C_(24) of the side-chain is not derived from acetate.
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