Abstract
To improve the design and performance of anoxic phosphorus uptake process, the biomass fractions of PAOs grown in anoxic environment and denitrification rate, with PHAs as carbon source, was investigated in a pilot scale study of EBPR system which was based on stoichiometry and mass balance. The system, containing Modified University of Cape Town (MUCT) reactor, was maintained at steady state with COD loading of 0.25 kg COD/(kg MLVSS d), and solid retention time (SRT) of 12 d. Based on mass balance calculation, 46.74% of organic matter, 50.95% of nitrogen, and 42.03% of phosphorus in the influent were converted to carbon dioxide, nitrogen gas, and poly-phosphate, respectively. In the second anoxic stage, the biomass yield of PAOs with nitrate as electron acceptor was 4.90 gC5H7O2N/d, and its yield fraction was 20.63%. For the reaction to proceed properly, 10.48 g PHA/d of PHAs was oxidized, and 3.68 g NO3--N/d of nitrate was reduced. The denitrification rate with PHAs as carbon source was 0.058 kg NO3--N/(kg MLVSS·d). These results showed that a higher amount of PAO growth under anoxic condition was observed in the MUCT system, and 0.058 kg NO3--N/(kg MLVSS·d) of the denitrification rate with PHAs could be used as the reference values for calculation of anoxic stage volume.
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