Abstract
Callus cultures of tobacco, pearl millet, and centipede grass do not survive when inoculated with the free-living, nitrogen-fixing bacterium Azospirillum . The rapidly multiplying bacteria overgrow the callus, and death of the plant tissues ensues in about a week after inoculation. However, so long as the callus cells survive, the bacteria show nitrogenase activity if the callus is being grown on nutrient media totally or largely devoid of combined nitrogen. Under similar conditions, sugarcane callus cultures grow for periods of more than 18 months with regular sub-culture, and considerable amounts of nitrogenase activity can be demonstrated in such associated cultures. Plantlets have been regenerated from sugarcane callus tissues inoculated with Azospirillum .
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