The Biology Activities of Isonicotinohydrazide Derivatives as an Anti-tuberculosis Candidate

Abstract

The compound N’-(3-chlorobenzoyl)isonicotinohydrazide, N’-(3-bromobenzoyl) isonicotinodyrazide, N’-(4-fluorobenzoyl)isonicotinohydrazide are isonicotinohydrazide derivative compounds which have antibacterial activity against bacteria Staphylococcus aureus, bacteria Bacillus subtilis as gram-positive Escherichia coli as gram-negative, and against strain Mycobacterium tuberculosis H37Rv. The purpose of this study was to obtain antituberculous candidates from isonicotinohydrazide compounds. The method used to test the antibacterial activity was the disc diffusion while for antimycobacterium using method Resazurin Microtiter Assay (REMA). The results of the antibacterial activity test on the bacteria Staphylococcus aureus compound N’-(3-chlorobenzoyl)isonicotinohydrazide had the lowest MIC value (0.26 ppm), while the bacteria Bacillus subtilis and Escherichia coli compound with the lowest MIC value of N’-(3-bromobenzoil)isonicotinodyrazide with a MIC value of 0.30 and 0,24 ppm. In the bacterium M. tuberculosis, H37Rv compound isoniazid has the smallest MIC value (3.125 ppm).