Abstract
This study was aimed to investigate microbiological and molecular markers of periodontal problems and its association with conception in 101 Iraqi women, 50 primary infertile while 27 secondary infertile and 24 pregnant as control group. Major periodontal pathogen in the subgingival plaque and cervical vaginal swab specimens was detected, and molecular marker (16SrRNA) gene used for detection of Fusobacterium in our study. Fusobacterium sp. isolates from oral samples were examined biochemically and confirmed by molecular detection, they were also subjected to antibiotic sensitivity test and were shown to be resistant to β-lactamase family of antibiotics and were mostly resistant to Ciprofloxacin. Four Fusobacterium sp samples were identified in both Oral samples and vaginal-cervical specimens of the same four women from the primary infertile group. Fusobacterium sp. was significantly (P<0.01) more frequently detected in the Oral samples and vaginal-cervical specimens among primary infertile group (38.7%) than pregnant (9.1%) as so among secondary (30.0%) than pregnant (9.1%). Analysis identified a model of a significant predictive factor of presence of Fusobacterium sp.: primary infertile [Odd ratio (OR) = 6.3 at 95% CI = 1.33-25.1] and secondary infertile OR = 4.2 at 95% CI = 1.84-12.22. this can show that infertalty state can be more risked than pregnant women to have the Fusobacterium sp and be effected by it.
Highlights
Fusobacterium, is a Gram negative bacterium, non-spore former, anaerobic species of the Fusobacteriaceae family that consists of nine genera including Fusobacterium and Leptotrichia
The Fusobacterium is a non-motile, rod-shaped, or bacilli bacterium. It shows a significant role in the progress of biofilm dental plaque as a bridge bacterium interaction with early and late colonizing bacteria in the oral cavity [3], as well as its role as a part of oral plaque, through its adhesive abilities which provide benefit as a link between early and late colonizers of this biofilm [27]
It is one of the species found in the oral cavity, in both unhealthy and healthy humans. It is found in different kinds of periodontal diseases including the simple reversible kind of gingivitis and the progressive irreversible forms of periodontitis: chronic periodontitis and localized aggressive periodontitis [33]
Summary
Fusobacterium, is a Gram negative bacterium, non-spore former, anaerobic species of the Fusobacteriaceae family that consists of nine genera including Fusobacterium and Leptotrichia. The Fusobacterium is a non-motile, rod-shaped, or bacilli bacterium It shows a significant role in the progress of biofilm dental plaque as a bridge bacterium interaction with early and late colonizing bacteria in the oral cavity [3], as well as its role as a part of oral plaque, through its adhesive abilities which provide benefit as a link between early and late colonizers of this biofilm [27]. It is one of the species found in the oral cavity, in both unhealthy and healthy humans. One research has already shed light on the effect of microbiological markers of periodontal infection on conception [38].This study has been applied with a cohort of young women who failed to get successful conception and aimed to find a connection between the oral microorganisms and conception by using microbiological and molecular methods
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