Abstract
Abstract The titer of a given antitoxin may vary with the toxin used in the assay. Most freshly prepared toxins, irrespective of methods of preparation, and one crystallized toxin gave similar titers with one antitoxin. Toxins which gave divergent titers were slightly turbid.These were very old or had been treated with chloroform. In one instance, a toxin which when fresh behaved normally, on ageing changed so as to give the test antitoxin a higher titer. It has been shown that variation in titer could be due to variations in the dissociation of toxin-antitoxin complexes. An empiric formula has been developed to adjust tetanus antitoxin titration values. When one antitoxin was titrated, application of this formula gave fairly consistent titers irrespective of the toxin used.
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