Abstract
Bioactive isolation was performed on the stem bark of Datuan (Ficus vasculosa Wall. Ex Miq), and extraction was carried out via the maceration method using acetone as a solvent. Furthermore, an attractant bioactivity test was conducted on acetone extract, A-G fraction, and composition of the isolates. The separation and purification via column chromatography produced a D8.3.5.7 fraction in the form of needle crystal of about 50 mg, at a melting point of 136°C–138.7°C. Thin-layer chromatography (TLC) analysis showed a single spot at an Rf value of 0.57 (n-hexane eluent: ethyl acetate 7:3), 0.36 (DCM eluent), and 0.24 (CHCl3 eluent). The isolated compounds were identified using infrared and UV–Vis spectrophotometry, as well as mass spectrometry. The characterization of the infrared spectrum of the isolated compound showed a strong OH goo band at 3461 cm-1 region and the absorption band at 2936.25 cm-1 exhibited a stretch of CH alkanes. These two bands are supported by the vibration at 1378.47 and 1462.55 cm-1 for CH absorption of methyl and methylene. The absorption band in the 1622 cm-1 region showed a stretch of conjugated C=C double bond, which is supported by absorption at 918.96 and 966.22 cm-1 as C–H alkene. The UV–Vis spectrophotometry showed absorption at λmax 263.97 nm A = 0.483, which was the result of electronic transition π → π*, and at λ 331.0 nm A = 0.274, which was an electronic result of n → π*. Meanwhile, identification via mass spectrometry that produces isolate has a molecular weight of 414.1 m/e with the formula C29H50O. Therefore, the bioactivity test results on compound D8.3.5.7 had an attractant activity of 71.67% against warehouse pests (Sitophilus oryzae L.) and an interest index of 0.63.
Highlights
Ficus is a plant genus from the family of Moraceae and order Morales, commonly found in tropical and subtropical areas worldwide.1,2 Over 500 species of this plant can be found in Indonesia and are widespread around Sumatera (102 species), Kalimantan (106 species), Papua (99 species), Sulawesi (83 species), Java (75 species), Maluku (71 species), and Nusa Tenggara (36 species).3 In general, the plant is a large woody tree that grows at a height of 30–50 m with a diameter of 90–110.1 The plant is usually harvested for dry and fresh consumption, especially its fruit, wood, bark, root, and leaves
The present study aims to obtain bioactive compounds in the stem bark of the Datuan plant (FvWM)
Crude Acetone Extract: About 4 kg of dried flour and approximately 30 g of concentrated extract of stem bark were obtained from 8 kg wet-stem bark of the Datuan plant (FvWM)
Summary
Ficus is a plant genus from the family of Moraceae and order Morales, commonly found in tropical and subtropical areas worldwide. Over 500 species of this plant can be found in Indonesia and are widespread around Sumatera (102 species), Kalimantan (106 species), Papua (99 species), Sulawesi (83 species), Java (75 species), Maluku (71 species), and Nusa Tenggara (36 species). In general, the plant is a large woody tree that grows at a height of 30–50 m with a diameter of 90–110.1 The plant is usually harvested for dry and fresh consumption, especially its fruit, wood, bark, root, and leaves. Ficus is a plant genus from the family of Moraceae and order Morales, commonly found in tropical and subtropical areas worldwide.. Over 500 species of this plant can be found in Indonesia and are widespread around Sumatera (102 species), Kalimantan (106 species), Papua (99 species), Sulawesi (83 species), Java (75 species), Maluku (71 species), and Nusa Tenggara (36 species).. The fruits of some species are edible and used as medicine. Many Ficus species are hosts to insects or warehouse pests that secretes resinous substances. They have some important components that are obtained for consumption or used as traditional medicine, bioactive compound, antioxidant, and pharmacological agent for human health improvement1.4,5
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