Abstract
<p><em>Monascus purpureus is a mold capable of producing pigment as secondary metabolites. Pigments of Monascus purpureus isolated from rice fermented solid known as red yeast rice. This study was conducted to</em><em>Monascus purpureus know the potential in generating new compounds of pigment produced by solid fermentation process with rice as a substrate. </em><em>The results indicate insulation compounds and new compounds of pigment Monascus purpureus. Beginning with an analysis of Thin Layer Chromatography (TLC) using the mobile phase n-hexane and ethyl acetate (</em><em>7</em><em>:3) with a Rf value obtained was 0.71. Analysis with UV-Vis spectrophotometry against red yeast rice extract aims to determine the maximum wavelength. The results of the analysis of UV-Vis spectrophotometry obtained one peak an absorption at 0.255 with a wavelength of 486 nm which showed that the red pigment monascorubramin compounds in Monascus purpureus. Isolation of Monascus purpureus pigments by Preparative Thin Layer Chromatography (Preparati</em><em>ve </em><em>TLC) using the mobile phase n-hexane and ethyl acetate (7:3). Results isolation pigment Monascus purpureus form of a ribbon that can fluoresces atλ 365 nm UV light with a Rf value obtained was 0.73 were further identification of the molecular weights of compounds Monascus purpureus using HPLC instruments with detectors MS (Mass Spectrometer). The results of LC-MS analysis of a sample INACC-F01 showed a new compound which has a molecular weight of</em><em> 357.41 and based on the literature that the </em><em>same </em><em>compounds </em><em>with </em><em>molecular weight is Monascopyridine C which is a derived compound from Monascus red pigment that is monascorubramin and rubropunctamin.</em></p><p><strong> </strong></p><p><strong><em>Keywords:</em></strong><em>Monascus purpureus, </em><em>pigment</em><em>, angkak, </em><em>Preparative TLC</em><em>, LC-MS</em><em>.</em><em></em></p>
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