Abstract
The intermediate filament-lamina-nuclear matrix system (IF-lamina-Nm System) can be shown clearlyusing selective extraction, embedment-free electron microscopy and whole mount cell preparation. A del-icate intermediate filament network was demonstrated in vaccinia virus factory for the first time. It isa part of the cytoplasmic intermediate filament network. The components and substructures of vacciniavirus bind firmly to intermediate filaments. Both assembled and assembling vaccinia viruses are fixedin the intermediate filament network. Direct connections between viruses and intermediate filaments canbe observed. We propose that vaccinia virus assembly depends on intermediate filaments as support.
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