Abstract
BackgroundThe current study investigates the effect of transcription factor Prox1 on the proliferation, migration, and invasion ability of lung cancer.MethodsLung cancer cell lines (A549 and H446 cells) were transfected with Prox1NAD and siRNA, respectively. Thus, the A549 and H446 cells overexpressed Prox1 after transfection of Prox1NAD plasmids, and A549 and H446 cells have low expression of Prox1 after transfection with siRNA. Reverse transcriptase quantitative PCR and western blot analyses were used to detect Prox1 mRNA and protein expression in cells. Plate clone formation experiments and MTT experiments were used to detect cell proliferation. Western blot was used to detect the expression of Rho family-related proteins in cells.ResultsCompared to untransfected wild-type A549 and H446 that served as blank controls, the expression level of Prox1mRNA and protein in A549 and H446 cells overexpressing Prox1 after plasmid transfection was high, while the expression level of Prox1mRNA and protein in A549 and H446 cells with low expression of Prox1 after siRNA transfection was low. With the increase of Prox1 expression, the expression of RhoA and RhoC increased, while the expression of RhoB decreased.ConclusionThe finding of this study may provide a new approach for the treatment of lung cancer using targeted gene therapy.
Highlights
Lung cancer is the most common primary malignancy of the lung, and it is currently the most common type of highly heterogeneous malignancy in the world [1,2]
Prior to investigate the effect of Prox1 on the migration and invasion of lung cancer cells, we firstly confirmed the expression of Prox1 in A549 and H446 cells overexpressing Prox1 after plasmid transfection
This study used the method of plate cloning to detect the clone formation of A549 and H446 cells overexpressing Prox1 after plasmid transfection and wild-type untransfected A549 and H446 cells
Summary
Lung cancer is the most common primary malignancy of the lung, and it is currently the most common type of highly heterogeneous malignancy in the world [1,2]. According to the 2009 data released by the National Cancer Center and the Bureau of Disease Control and Prevention of the Ministry of Health in 2012, lung cancer ranked first in the global cancer incidence [4]. In the past few decades, progress has been made in various treatment methods, such as drug therapy, radiation therapy, and targeted gene therapy, and the overall survival rate of lung cancer has improved, lung cancer is still the leading cause of health threats, and so the research on the pathogenesis of lung cancer is still a hot spot [5,6]. Current research has shown that Prox is overexpressed in tumor tissues, such as kidney cancer and gastric cancer, while downregulated in tumor tissues, such as primary liver cancer, indicating that it may play different roles in different tumor tissues [11]. Prox is implicated in the growth and progression of cancer and has been linked in different
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