Abstract
AbstractPolymerase chain reaction (PCR) assay, restriction fragment length polymorphism analysis, sequencing of PCR‐amplified cDNA, graft transmission and mycological tests were used to investigate the possible association of phytoplasma infection with rose dieback symptoms and to identify any associated pathogen. Rose plants with shoot dieback symptoms, symptomless rose plants, and experimentally infected periwinkle seedlings were shown to be infected with phytoplasma, which was classified to the aster yellows (AY) group, subgroup 16SrI‐B, based on the analysis of the nucleotide sequence of a 1101‐bp fragment of 16S rRNA gene (GenBank accession no. AF450325). No evidence was obtained for the infection of rose plants with Verticillium albo‐atrum. The rose plants cultivated under plastic cover without heating or under outdoor conditions, in contrast to greenhouse‐grown roses, developed downy mildew symptoms caused by Peronospora sparsa.
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