Abstract
Abstract Administration of a physiological dose of radioactive vitamin D3 to vitamin D-deficient chicks results in a localization of the radioactivity isolated from intestinal mucosa within the nuclear chromatin fraction. Extraction and chromatography of this chromatin-bound radioactivity in several systems indicates that 87% of it exists as a polar metabolite of vitamin D3. This polar metabolite has biological activity equivalent to the parent vitamin. The association of this metabolite with the chromatin fraction occurs only in the target intestinal mucosa and is specifically inhibited by pretreatment of the rachitic chick with nonradioactive vitamin D3 or vitamin D3 analogues such as vitamin D2 and dihydrotachysterol3. The time course of appearance of the polar metabolite in the entire intestine parallels the location of radioactivity in the chromatin fraction and is consistent with the lag in the physiological response to vitamin D.
Highlights
Administration of a physiological dose of radioactive vitamin DB to vitamin D-deficient chicks results in a localization of the radioactivity isolated from intestinal mucosa within the nuclear chromatin fraction
Reported that, after a physiological dose of 3H-vitamin D, the tritium isolated from the intestinal mucosa of vitamin Ddeficient chicks is located primarily in t’he nuclear fraction [12]
The radiochemical purity of these radioactive steroids was assessed by chromatography in a reversed phase column system which is capable of separating vitamin Dz from vitamin D3 [17]
Summary
Administration of a physiological dose of radioactive vitamin DB to vitamin D-deficient chicks results in a localization of the radioactivity isolated from intestinal mucosa within the nuclear chromatin fraction. Reported that, after a physiological dose of 3H-vitamin D,, the tritium isolated from the intestinal mucosa of vitamin Ddeficient chicks is located primarily in t’he nuclear fraction [12] This finding was cited as possible evidence for the interaction of the vitamin or its metabolites with nuclear factors controlling or initiating protein synthesis. One approach in assessing the relationship of these metabolites to the physiological response characteristic of vitamin D is the isolation of the receptor molecule or molecules or site for the active form of the vitamin within the target organ In this communication we wish to document further the nuclear localization of radioactivity following a physiological dose of radioactive vitamin D and to present data which demonstrate that a biologically active metabolite of vitamin Da is bound to intestinal mucosa chromatin. $ Recipient of United States Public Health Service Training Grant l-TOl-ES-00084-01
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