Abstract
The feasibility of using renewable natural gas for ammonia stripping in anaerobic digestion of poultry manure application was investigated and compared with ammonia stripping with air. Renewable natural gas and air led to comparable ammonia removal efficiencies of up to 60 and 69 % under elevated pH and temperature (9.5 and 55 °C), respectively. The consequential improvement from these treatments on biogas production was 58 and 70 % for samples treated with renewable natural gas and air, respectively. Shotgun metagenomic microbial analysis revealed that the hydrogenotrophic pathway was responsible for most biogas production as the archaeal relative abundance of Methanoculleus bourgensis was about 95 % in all digestates. Moreover, ammonia stripping did not impact the diversity of the microbial consortia but decreased the inhibitory effects on hydrogenotrophic archaea.
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