Abstract
Tobacco is ground and extracted with chloroform, and thin-layer chromatography is used to separate the 3 major tobacco alkaloids—nicotine, nornicotine and anabasine. Each of the alkaloids is quantitatively removed by scraping the spot into a container and dissolving in ethanol-sulfuric acid. A small volume of the ethanol solution is then measured phosphorimetrically. Excellent recoveries of each of the tobacco alkaloids were obtained; the relative standard deviation of analysis was 6% or less for all analyses. The total time for analysis of the 3 alkaloids was less than 90 min. This method is considerably faster and more accurate than previous methods.
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