The application of stem cells, visible and infrared light in regenerative medicine. Part 1

Abstract

The present article was designed to overview the experimental studies of visible and infrared light irradiation of human and animal stem cells (SC) in vitro and in vivo for the evaluation of its photobiomodulatory effects. The results will be used to elaborate substantiation for the choice of the parameters of SC light irradiation and to develop recommendations for the application of this method in regenerative medicine (RM). The clinical application of light irradiation is a matter of contrsy, in the first place due to the difficulties encountered in the rational choice of irradiation parameters. The theoretical substantiation of such choice remains a stumbling block too despite the long history of photoghromotherapy. There is thus far no reliable theoretical basis for the adequate choice of such irradiation parameters as power density, radiation dose, and exposure time. The experiences with the light application for the purpose of regenerative medicine have never been summarized. The present review encompasses 78 articles selected for the basic analysis that report the studies with the use of a variety of SC types. The analysis has demonstrated that clinical investigations into the influence of light on the stem cells are still in their infancy. It was shown that the irradiation parameters need to be chosen taking into consideration the type of the stem cells. Different authors report the achievement of the maximum SC proliferation and differentiation rates at energy densities as high as 50 mW/sq.cm, small radiation doses (around 1 J/sq.cm) and exposure time (on the order of seconds). The general conclusion for Parts 1 and II of this communication will be presented in the next issue of this journal (number 2, 2015).