The Application of Modern Biotechnology in Protein Interaction Research-A Review

Zi-Yue Liu,Meng-Yang Zheng,Xin-Xiang A,Fang Yang,Hui-Long Li,Bo Peng,Wei Zhou,Juan Peng,Yan-Yang Sun,Xia-Yu Tian,Rui-Hua Pang,Ya-Qin Huang,Yan-Ming Zhang,Hongyu Yuan,Xiao-Hua Song,Quan-Xiu Wang

doi:10.32861/jac.72.39.47

Abstract

Intermolecular interaction is the material basis for cells to achieve their functions, and protein-protein interaction is an important approach to illuminate the regulation network of biological molecules and has important theoretical significance and potential application value for revealing the activity law of life in nature. This paper mainly summarizes and analyzes the new advances and applications of modern biotechnologies in the study of protein-protein interactions, including local surface plasmon resonance (LSPR), yeast two-hybrid, GST-Pull-down, bimolecular fluorescence complementation, and coimmunoprecipitation. At the same time, the principles of different research methods for protein-protein interaction and their other applications in the field of life sciences are also discussed, all of these will provide a reference value for the analysis of protein-protein interaction and the molecular regulation mechanism of biomacromolecules.

Highlights

The Inner Niger Delta or DIN has been recognized as a Ramsar site since February 1, 2004
The specialized services of the State intensify the chemical treatment during the return of the evening when the birds have a great flight activity and are eager to group in their dormitories or nesting areas or in the morning of happiness when they leave
For foraging the bird's contact with the drops may be by sedimentation or by impact

Summary

Introduction

The Inner Niger Delta or DIN has been recognized as a Ramsar site since February 1, 2004. It is so important for people practicing agriculture, fishing, and breeding as well as the animals that live there because of the diversity and the richness of its ecosystem. The primary sector (agriculture, livestock, fishing) is the primary source of economic growth and employs over 80% of the population. Some of which are residents, and others seasonal or migratory, have habitats very suitable for their feeding and reproduction. 1963 presented an inventory of DIN avifauna composed of several species including residents and migrants. The granivores present in the area constitute real dangers for cereal crops. The peasants tirelessly engage in the means of struggle against them

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