Abstract
The applicability of immobilized silicone phases, as reversed phase retentive layers in 5–25µm fused silica capillaries for open tubular liquid chromatography (OTLC) has been investigated. Various types of silicone phases have been tested, of which vinyl containing gums show the most promising features, to create a stable retentive layer in fused silica capillaries. Diffusion coefficients of solutes in the immobilized silicone phases were determined by static measurement and found to be in the order of 5–27 · 10−12 m2/s. These relatively small diffusion coefficients form the main drawback of the immobilized silicone phases, because this seriously hinders the use of thick layers of the stationary phase which is preferred in OTLC to avoid mass overload.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
