The Application of Different Probiotics for Prevention of Motile Aeromonas Septicemia Disease on African Catfish (Clarias gariepinus)

Abstract

Motile Aeromonas Septicemia has become a problem in aquaculture. This study set out to investigate the usefulness of the probiotic Lactobacillus acidophilus and Staphylococcus lentus on survival rate, growth, and feed conversion efficiency of African Catfish (Clarias gariepinus). In vitro test was carried out to evaluate the effectiveness of probiotic against A. hydrophila and followed by in vivo test. In earlier treatment, Aeromonas hydrophila (final concentration 104 CFU mL−1), L. acidophilus, and S. lentus at a final concentration of 104 CFU mL−1 were added in aquaria every 2 days. there are four of the aquaria with three replicates, one control (without probiotic). In vitro test exposed the inhibition zone of L. acidophilus, S. lentus, and the combination of both bacteria was 11.72±0.55, 13.03±2.99, and 14.66±1.50 mm, respectively. After 21 days of treatment, the addition of the probiotic significantly increased the survival rate (P<0.05) for all conducts compared with controls. In vivo test showed that the application of probiotics in media of cultivation could reduce the number of A. hydrophila, improve the specific growth rate (SGR 6.00 ± 0.05%), and also increase the survival of catfish compared to the positive control. The results indicated that the combination of probiotics L. acidophilus and S. lentus appearances the highest survival (91.11 ± 1.53 %) of all treatments and the ratio of feed conversion was found to be decreased (0.23). This study highlights the combination of L. acidophilus and S. Lentus that could prevent MAS disease on Catfish.