Abstract
A preliminary study on the applications of UV-visible derivative spectroscopy to forensic toxicology has been conducted. The presentation of data in the derivative mode has considerable potential as an aid to the identification of compounds with very similar UV-visible spectra. The value of second-derivative spectra for the elimination of broad background absorbance is demonstrated using a liver extract containing a low concentration of amphetamine. Two commercial UV-visible spectrometers have been used which employ different methods of obtaining derivative spectra (electronic differentiation and numeric differentiation) and the limitations due to wavelength shifts produced by the former are discussed.
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