Abstract
Several types of liquid—liquid system were tested as phase systems in high-performance liquid chromatography. The stationary phase is generated on prepacked silica columns by pumping through the mobile phase which is presaturated with the stationary phase ( in situ coating technique). The amount of stationary phase generated, the phase ratio and the reproducibility of retention data were determined. The effect of the column and reservoir temperature on the phase ratio and retention was extensively investigated. Although in-situ-generated liquid—liquid systems need more care than other common liquid chromatographic phase systems, such as extensive presaturation of the mobile phase and precise thermostatting of the stock reservoir and column, the results demonstrate that such systems are applicable in high-performance liquid chromatography. This is illustrated with a number of separations of text mixtures of various classes of compounds.
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