Abstract
Two trials in vivo and in vitro were conducted, in vivo to determine the apparent digestibility of gross energy, crude protein, dry matter, acid detergent fiber, neutral detergent fiber and apparent digestible energy in 10 corn by-products. In vivo the diets included one basal corn diet, four corn gluten meal diets, four corn distillers dried grains with solubles diets and two corn distillers dried grains diets using the different methods, 12 crossbred barrows weigh 40±1.6 kg were allocated into individual metabolic crate, according to a 6×6 Latin square design. In vitro using flask technique, filter bag technique and dialysis tubing technique, the digestibilities of gross energy, crude protein and dry matter in corn gluten meal and corn distillers dried grains with solubles were investigated. Pepsin, pancreatin, intestinal fluid, rumen fluid and cellulase were used in incubation. The results showed that correlation coefficient was 0.73 in corn distillers dried grains with solubles between the digestibility of crude protein and acid detergent fiber in vivo (p<0.01); and correlation coefficient was 0.68 in corn distillers dried grains with solubles between the digestibility of gross energy and neutral detergent fiber in vivo (p<0.01). Apparent digestible energy (DE) of corn by-products in pig total tract was predicted by the percentage of crude protein (CP) and the content of gross energy (GE) in feedstuff. The equation: DE=5,601.09+26.69×CP %-0.5904×GE, (R 2 =0.72). In vitro, filter bag technique was more convenient; furthermore, the digestibility for the treatments (pepsin+pancreatin+rumen fluid and pepsin+pancreatin+cellulase) was better. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 3 : 379-385)
Highlights
Corn by-products are valuable protein sources for animals as unconventional feedstuffs
Pancreatin+rumen fluid and pepsin+pancreatin+cellulose : The trial steps were respectively similar to that of the flask technique, except that the sample, enzyme and buffer were added into the dialysis tubing, the entrances of dialysis tubing with sample, enzyme and buffer were sealed by cord, the dialysis tubing was put into a bottle (500 ml volume), which contained 40 ml phosphate buffer
Corn gluten meal came from corns after the extraction oil and starch; dried grains with solubles (DDGS) and dried grains (DDG) came from corns after extraction oil and alcohols, the process techniques were different between DDGS and DDG
Summary
Corn by-products are valuable protein sources for animals as unconventional feedstuffs. DDGS and DDG come from corns by the extraction of oil and alcohol using different processing techniques. An in vitro method with a general validity for all kinds of feedstuffs should be established to measure the digestibility of nutrient. Two trials were conducted to investigate correlations of the nutrient digestibility in vivo and in vitro in this study. Trial 2 was designed to apply multienzyme systems for investigating the in vitro digestibility of CP, DM and GE of corn by-products. Data from both trials were collected and used to find the correlations among digestibilities of CP, GE, ADF, NDF, DM and DE in corn by-products
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