Abstract
Oxidation reactions on foods and drugs can be inhibit by using antioxidants that binding to free radicals and reactive molecules. The Ambonese banana (Musa paradisiaca) peel and orange (Citrus reticulata) peel have antioxidant activity potency. The aim of this research is knowing the antioxidant activity of combination Ambonese banana (Musa paradisiaca) peel and orange (Citrus reticulata) peel. IC50 for antioxidant activity potency between Ambonese banana (Musa paradisiaca) peel and orange (Citrus reticulata) peel is expected to increase the antioxidant activity if used as combination that can inhibit oxidation reaction. The inhibit concentration of antioxidant activity is indicated by IC50. The compound that has the highest antioxidant activity will show lowest level of IC50. The antioxidant activity test was performed by DPPH method using UV-Vis spectrophotometer on 516 nm and color change of DPPH from purple to yellow when both of them react with antioxidant compound. The results of IC50 of banana peel extract was 114 mcg / mL; IC50 of orange peel extract was22.2 mcg / mL. The greatest inhibition of the combination of both extracts was 73.53% consisting of 25 μL (0,005%) sample of tangerine orange peel extract and 75 μL (0,015%).The biggest inhibition of the combination of both extracts was 73.53% consisting of 25 μL (0.005%) samples of tangerine orange peel extract and 75 μL (0.015%).
Highlights
The content of vitamins and minerals contained in the diet is responsible for the health of the body
Based on the above review, research is needed to investigate the antioxidant activity of banana peel extract and orange peel extract which is expected to have higher antioxidant activity
The mixture reaction (5 ml) contains total volume combination of banana and orange peel extract was 100 μL, 1 mL of 0,4 Mm DPPH (15,7 mg DPPH in 100 mL of ethanol absolute) and added ethanol absolute up to mark and made various concentrations of test solution dissolved in ethanol absolute
Summary
The content of vitamins and minerals contained in the diet is responsible for the health of the body. Orange peels have the potential of antioxidant activity. According to research Tumbas et al, (2010), orange peel (Citrus reticulata) has antioxidant activity with IC50 0,179 mg/mL [1]. Bananas contain flavonoid compounds and other phytochemical compounds so they have potential antioxidant activity (Imam et al, 2011) [4]. Research Atun et al, (2007) showed the results that banana peel methanol extract contains compounds that have antioxidant activity [5]. Banana peel contains a variety of substances, including vitamin A and carotenoid substances that function as antioxidants. Based on the above review, research is needed to investigate the antioxidant activity of banana peel extract and orange peel extract which is expected to have higher antioxidant activity
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