The Antimicrobial of Selenium Nanoparticles and Combination with Cinnamon Oil Against Salmonella Typhi Which Cause Diarrhea in Human

Abstract

Many bacterial species can cause diseases in humans, such as the bacteria that cause diarrhea as a result of having genetic characteristics that can form pathogenic factors, which help the bacterium to produce harmful effects in the host's body. From (60) fecal samples of diarrheal cases of human, Salmonella spp.42(70%) where Salmonella typhi consist29(48.33%) isolates and Salmonella paratyphi consist13(21.67%) isolates, E. coli 10(16,66%), Enterobacter 4(6,66%) and Shigella 4(6,66%). On Congo red agar (CRA) plates, among (29) S. typhi bacterial isolates from human, all the isolates (100%) were showed biofilm formation through formation of brown colonies with a dry crystalline quality. Antibiotic susceptibility profile of Salmonella typhi. isolated from fecal sample of human were determined by using Vitek2 system, the results revealed that Salmonella typhi were100% resistant to Ticarcillin and 89.7% to Aztreonam while the rest of the antibiotics were less resistant. Selenium nanoparticles (Se-NPs) are green synthesized using cinnamon extract. The antibacterial activity of the cinnamic ethanolic extract and synthesized SeNPs was investigated against isolates of human source Salmonella typhi by using the agar well diffusion method and tested by using different concentrations (25,50,100 μg/mL).According to the data, cinnamon extract only produced an inhibition of 10.66 mm at the same dose of 100 mg/ml, but Se-NPs produced a good inhibition at that concentration of 14.33 mm.