Abstract
In this study, the anticoagulation effect of glycosaminoglycan from <em>Mactra veneriformis</em> was studied. The results showed that glycosaminoglycan mainly exerted anticoagulation via antithrombin III. Glycosaminoglycan could passivate the function of heparin cofactor II inhibiting thrombin activity. Glycosaminoglycan significantly reduced the activities of coagulation factor II, V, VII, X, VIII, IX, XI, XII as well as fibrinogen content in the plasma (p<0.05, p<0.01). Besides, glycosaminoglycan could extend blood recalcification time in rats by shielding Ca<sup>2+</sup> in plasma and significantly reduced Ca<sup>2+</sup> concentration in rats and mice serum (p<0.05, p<0.01). Glycosaminoglycan reduced the Ca2+ concentration in serum in a more intensive way than that of heparin sodium (p<0.05, p<0.01).
Highlights
Mactra veneriformis belongs to Mollusca, Lamellibranchia, Mactridae and it mainly inhabits in the intertidal and subtidal zone and neritic mud
Glycosaminoglycan (1:10) was added in standard plasma, Antithrombin III (AT-III) deficient plasma, HC-II deficient plasma and both factor-deficient plasma respectively, normal saline was added in the control group and the concentration of glycosaminoglycan was 4 mg/mL
We found that glycosaminoglycan from M. veneriformis could significantly extend Thrombin Time (TT) and Prothrombin Time (PT) in standard plasma and HC-II deficient plasma (p
Summary
Mactra veneriformis belongs to Mollusca, Lamellibranchia, Mactridae and it mainly inhabits in the intertidal and subtidal zone and neritic mud. It is a common economic shellfish in Chinese coastal areas. Theories of traditional Chinese medicine describe clams as cold and salty flavor, which can nourish yin, facilitate dieresis, reduce phlegm and soften hard mass. Studies have showed that glycosaminoglycan from M. veneriformis possessed good hypoglycemic and antioxidation effects, as well as certain anticancer effect (Cui et al, 2013; Yuan et al, 2011; Chang et al, 2009). Recent research proved that glycosaminoglycan from M. veneriformis presented excellent resistance to rats’ deep venous thrombosis as well as anticoagulation in vitro and vivo (Cui et al, 2014). We investigated anticoagulation effect of glycosaminoglycan from aspects like heparin cofactors, coagulation factors and Ca2+.
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