The Anticancer Effects of Hexane Extract of Rainbow Trout (Oncorhynchus mykiss) Muscle Enriched with Vegetable Oil Brasica sp.

Abstract

Background: As Canola oil (Brasica sp.) has beneficial compositions of essential fatty acids like saturated fatty acids, mono unsaturated fatty acids (MUFA) and poly unsaturated fatty acids (PUFA), practically it use in many experimental purposes. There are considerable amounts of oleic acid which is mono unsaturated fatty acids in canola oil that caused a good resistance to thermal processes such as deep frying. Rainbow trout is the main fish species farmed in about 85% of the total rate allocated to producing aquaculture fish in Iran. Rainbow trout has found in food baskets of many people due to its high nutritional value. Methods: in this study the hexane extracts of fish muscle isolated with purity of 250, 50, 10, 5 and 0.5 μg/ml at regular intervals of 24, 48 and 72 hours to determine the effects of hexane extracts on parameters which are associated with the survival of skin cancer cells A431 by MTT method in vitro comparison. Results: The effect of various concentrations of hexane extracts from fish muscle showed that the extracts of muscle at varying time intervals in hexane extracts administration had different effects on mortality of A431 cancer cells. Increasing of hexane extracts had significantly decreased the carcinogenic cells. The concentrations of hexane extract in 250 μg/ml at 24 hours were killed 50 percent of skin cancer A431cell lines. The addition of Brasica sp. vegetable oil as a dietary supplement is required to fish diet rations. Conclusion: In the present study the hexane extract inhibit the growth of cancer cells where the extract can be used as an anticancer agent for the operation of this lineage.