The Antibacterial Effects of Apacaries Gel on Streptococcus mutans: An in vitro Study.

Apa Juntavee,Supaporn Chatchiwiwattana,Panta Treesuwan,Nartsajee Nualkaew,Jomjai Peerapattana,Ariya Ratanathongkam

doi:10.5005/jp-journals-10005-1241

Apa Juntavee, Supaporn Chatchiwiwattana + Show 4 more

Open Access

https://doi.org/10.5005/jp-journals-10005-1241

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Abstract

ABSTRACTBackground: New approaches for chemomechanical caries removal require effective materials with antibacterial properties for removal of infected dentin. Apacaries gel is a newly developed material comprised polyphenol from mangosteen extracts and papain mixed in gel preparation.Aim: This study evaluated the antibacterial effects of Apacaries gel on Streptococcus mutans in vitro.Materials and methods: Mangosteen pericarp powder was extracted. The amount of phenolic compounds was determined using the Folin-Ciocalteu method. The time-kill kinetics were investigated. Mangosteen extract and papain were mixed with gel base to develop Apacaries gel. The inhibition zone of the Apacaries gel was determined using agar well diffusion methods.Results: The mangosteen pericarp extract, which contains α-mangostin, was active against S. mutans strain ATCC25175. The time-kill kinetics curve showed that applying 1 mg/ml of mangosteen extract can reduce S. mutans by 50% within approximately 5 seconds; after this reduction, the bacterial count rapidly dropped to 0 within 60 seconds. Using mangosteen extract and papain mixture gel preparation resulted in a larger inhibition zone than using the mangosteen extract gel or papain gel separately.Conclusion: Apacaries gel can effectively inhibit S. mutans strain ATCC25175. Apacaries is capable of S. mutans inhibition better than both mangosteen extract or papain separately.How to cite this article: Juntavee A, Peerapattana J, Ratanathongkam A, Nualkaew N, Chatchiwiwattana S, Treesuwan P.The Antibacterial Effects of Apacaries Gel on Streptococcus mutans: An in vitro Study. Int J Clin Pediatr Dent 2014;7(2):77-81.

Highlights

In young children, the risk of caries has been shown to be greater when the colonization of Streptococcus (S. mutans) in the mouth occurs earlier.[1]
Using mangosteen extract and papain mixture gel preparation resulted in a larger inhibition zone than using the mangosteen extract gel or papain gel separately
Apacaries is capable of S. mutans inhibition better than both mangosteen extract or papain separately

Summary

Introduction

The risk of caries has been shown to be greater when the colonization of Streptococcus (S. mutans) in the mouth occurs earlier.[1] Dental caries is a major cause of pain and infection, which can have severe consequences for the quality of life of the affected children and their families.[2] Avoiding the early colonization of S. mutans may lead to favorable long-term effects on the caries experience and the need for restorative treatment.[3] A previous approach to the treatment of caries using hand excavation was a painful, ineffective and tedious method for caries removal.[4] Rotary instruments from low speed to ultrahigh speed evolved from that approach. Thermal and pressure effects on the pulp produced pain and were major drawbacks to rotary instruments.[5]. New approaches for chemomechanical caries removal require effective materials with antibacterial properties for removal of infected dentin. Apacaries gel is a newly developed material comprised polyphenol from mangosteen extracts and papain mixed in gel preparation

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