Abstract
Background: The nanoparticles synthesis through biological processes is evolving a new era of research interests in nanotechnology. In usual methods silver nanoparticles are synthesized through chemical methods, having extremely toxic and flammable natures. Objectives: The aim of the present study was to synthesize silver nanoparticles, through the green method of utilizing Sesamum indicum (S. indicum) extract and to determine the potential antibacterial effects of the product against multi-drug resistant Escherichia coli (E. coli). Materials and Methods: The formation and characterization of AgNPs (silver nanoparticles) were confirmed by UV-vis spectroscopy, energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD) and transmission electron microscope (TEM). All 30 strains of E. coli were isolated from urine cultures of hospitalized patients (Amir Al-Momenin Hospital, Zabol, South-Eastern Iran) with urinary tract infection, 2011-2012. The minimum inhibitory (MIC) concentrations were investigated by microdilution method. Results: The results showed that isolated E. coli were resistant to four different antimicrobial agents including ceftazidime (26.6%), cefixime (40%), tetracycline (63.3%) and erythromycin (56.6%). The highest MIC value for produced nano silver in S. indicum seed extract, was 200 ppm, against five isolates of E. coli. Conclusions: Considering the sufficient antimicrobial activities of nanoparticles tested in this study, they are suggested for enterobacterial infection treatment, especially in hospital environment.
Highlights
The nanoparticles synthesis through biological processes is evolving a new era of research interests in nanotechnology
Our results show that E. coli were resistant to four antimicrobial agents including ceftazidime (26.6%) cefixime (40%), tetracycline (63.3%) and erythromycin (56.6%) and the highest minimum inhibitory concentration (MIC) value was found to be 200μL/mL against five isolates of E. coli
Duran et al showed that cotton fabrics incorporated with silver nanoparticles, displayed a significant antibacterial activity against S. aureus [12]
Summary
The nanoparticles synthesis through biological processes is evolving a new era of research interests in nanotechnology. Objectives: The aim of the present study was to synthesize silver nanoparticles, through the green method of utilizing Sesamum indicum (S. indicum) extract and to determine the potential antibacterial effects of the product against multi-drug resistant Escherichia coli (E. coli). Results: The results showed that isolated E. coli were resistant to four different antimicrobial agents including ceftazidime (26.6%), cefixime (40%), tetracycline (63.3%) and erythromycin (56.6%). The highest MIC value for produced nano silver in S. indicum seed extract, was 200 ppm, against five isolates of E. coli. The development of resistant or even multi-drug resistant pathogens has become a major problem These problems and needs have led to a resurgence in the use of silver-based antiseptics which have broad-spectrum activity and considerably lower propensity to induce microbial resistance, compared to the antibiotics [1, 2]. Sesame seed oil is used in cooking, salad preparation and margarine and is the raw material for some industrial products including paints, varnishes, soaps, perfumes, pharmaceuticals and insecticides, while sesame seeds are used in baking, candy production and other food industries
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