Abstract
Background: Indonesia has many types of plants, which have medicinal properties and are used to cure various diseases. One of plant that has medicinal properties is the Angsana plant (Pterocarpus indicus Willd.) which traditionally can cure dental and oral diseases. Method: This research used experimental laboratory with disc diffusion methods. Angsana (Pterocarpus indicus Willd.) was taken from as city ornamental tree in Samarinda city. The bacteria which used were Streptococcus mutans. The samples consisted of 11 treatment groups is leaves ethanol extract, stem bark, and Angsana latex respectively with concentration 25%, 50%, 75%, positive control group (chlorhexidine 0,2%), and negative control group (sterile aquades). Data analysis using Shapiro-Wilk test and One-Way Annova test. Result: The results showed that leaves ethanol extract, stem bark, and Angsana latex (Pterocarpus indicus Willd.). All concentrations have antibacterial activity against the growth of Streptococcus mutans. The highest and the lowest concentrations that can inhibit the growth of Streptococcus mutans are 50% ethanol stem bark extract and 25% leaves ethanol extract. Conclusion: The conclusion of this study proves that the leaves, stem bark ethanol extract, and Angsana latex (Pterocarpus indicus Willd.) Have antibacterial activity against the growth of Streptococcus mutans.
Highlights
Indonesia has many types of plants, which have medicinal properties and are used to cure various diseases
Result: The results showed that leaves ethanol extract, stem bark, and Angsana latex (Pterocarpus indicus Willd.)
Conclusion: The conclusion of this study proves that the leaves, stem bark ethanol extract, and Angsana latex (Pterocarpus indicus Willd.) Have antibacterial activity against the growth of Streptococcus mutans
Summary
Penelitian ini merupakan penelitian eksperimental laboratoris dengan desain penelitian yang digunakan adalah the post test only control group design. Penelitian ini menggunakan media Muellerhinton Agar (MHA), Mueller-hinton Broth (MHB), blank disc dari OxoidTM berdiameter 6 mm, sediaan bakteri Streptococcus mutans (Standar ATCC), Chlorhexidine gluconate 0,2% dari MINOSEP®, etanol 96%, petridish, rotary evaporator, kertas saring Wathman® no.[42], digital caliper dari TRICLE BRAND®, spektrofotometer, kapas lidi steril, dan aquadest sterile. Penelitian ini menggunakan daun, kulit batang, dan getah Angsana (Pterocarpus indicus Willd.) yang tumbuh di sepanjang jalan Kecamatan Gunung Kelua Samarinda. Daun dan kulit batang Angsana (Pterocarpus indicus Willd.) masing-masing dicuci secara terpisah hingga bersih lalu dikeringkan selama 7 hari. Kulit batang dan getah yang sudah keringkan dimaserasi dengan pelarut etanol 96% selama 3 hari. Blank disc direndam ekstrak etanol daun, kulit batang, dan getah Angsana (Pterocarpus indicus Willd.) dengan masing-masing konsentrasi 25%, 50%, dan 75%, sebagai kontrol digunakan Chlorhexidine gluconate 0,2%, dan aquadest sterile. Uji statistik dengan Shapiro-Wilk, dengan nilai terdistribusi normal (p > 0,05) dan uji One-way Anova, dengan nilai signifikansi p > 0,05 tidak bermakna
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