Abstract
Background: An interaction has been reported between Nigella Sativa (NS) and ranitidine (RAN) on gastric ulceration induced by ethanol in rabbits; the combination NS and RAN caused disappearance of anti-ulcer effect of NS or RAN. Objective: to investigate interaction of NS with a proton pump inhibitor omeprazole (OMP) on ethanol induced gastric ulceration in rabbits. Methods: 24 mature rabbits were divided into 4 groups. The animals were fasted for 48 hours then treated as follow: group 1, 2, 3 and 4 were treated respectively with normal saline (oral), NS oil (10ml/kg) orally, OMP (20mg/kg) IP, and NS+ OMP. One hour later, animals were given absolute ethanol orally; and sacrificed 3 hours later for estimation of Ulcer index (UI), gastric pH, malondialdehyde (MDA), glutathione (GSH), histamine (HIS) levels in serum and gastric tissue. Results: Ethanol induced gastric ulceration in all animals with an UI of 10 ± 0.11 mm2. This effect was paralleled with reduction in gastric pH, increased MDA and HIS and reduction in GSH. UI was reduced to 5.13 ± 0.68 mm2 in NS group, P value = 0.07 and to around zero in OMP group. NS or OMP treatment resulted in reduction in serum and tissue MDA and HIS levels and increased in GSH and gastric pH levels. In NS + OMP treated group, UI became higher than OMP group with MDA and HIS tended to rise and GSH and gastric pH declined. Conclusion: NS + OMP diminished the gastro-protective effect of either NS or OMP.
Highlights
Nigella sativa (NS) is a medicinal plant increasingly used in practice either alone or in combination with many drugs for treating various health problems such as gastrointestinal, respiratory, metabolic, immune related and cardiovascular diseases[1,2], these effects were largely attributed to its antioxidant potential
There were numerous studies documenting an interaction between Nigella Sativa (NS) and some drugs such as sildenafil[6], phenytoin[7], cyclosporine [8], losartan [9] with a reduction in the area under the concentration time curve (AUC), Cmax, and bioavailability
OMP treatment achieved near complete inhibition of gastric ulceration with a mean Ulcer index (UI) of 0.67 ± 1.21 mm2 which was significantly lower than that obtained with the ethanol treated group (P = 0.002) and NS treated group (P = 0.001)
Summary
Nigella sativa (NS) is a medicinal plant increasingly used in practice either alone or in combination with many drugs for treating various health problems such as gastrointestinal, respiratory, metabolic, immune related and cardiovascular diseases[1,2], these effects were largely attributed to its antioxidant potential. [3] As reported with many drugs, herb-drug10.33762/mjbu.2020.127147.1018 interactions were widely studied and considered as a serious problem which may adversely affect patient's health.[4,5] There were numerous studies documenting an interaction between NS and some drugs such as sildenafil[6], phenytoin[7], cyclosporine [8], losartan [9] with a reduction in the area under the concentration time curve (AUC), Cmax, and bioavailability. In a previous study, Ahmed and colleagues[11] had reported an interaction between NS and ranitidine in a rabbit model of alcohol induced gastric ulceration; the study showed that the observed antiulcer effect with either NS or ranitidine was considerably decreased when the two agents were given together. The mechanism behind this interaction was not known. An interaction has been reported between Nigella Sativa (NS) and ranitidine (RAN) on gastric ulceration induced by ethanol in rabbits; the combination of NS and RAN caused disappearance of anti-ulcer effect of NS or RAN
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