The anti-inflammatory role of nuclear factor-kappa B essential modulator binding domain peptide in a rabbit osteomyelitis model

Abstract

Objective To investigate the anti-inflammatory role of nuclear factor-kappa B essential modulator binding domain (NBD) peptide in a rabbit osteomyelitis model.Methods Twenty-four male rabbits were used to establish models of chronic osteomyelitis with Staphylococcus aureus (ATCC25923).The animals weighed from 2 to 3 kilograms (average,2.5 kg).Three random rabbits were sacrificed 3 weeks later to verify successful modeling of chronic osteomyelitis by radiological,bacteriological and pathological observations.The remaining 21 successful models were randomized into 3 groups(n =7).The blank group received no further treatment,the control group received debridement,and the experimental group received debridement plus NBD peptide (3 mg/kg body weight).C reactive protein(CRP),erythrocyte sedimentation rate (ESR),leucocyte count,and HE staining of focus tissue were observed l to 6 weeks after intervention.Results At pre-intervention,and 1 to 6 weeks post-intervention,the CRP and ESR levels showed a decreasing trend in all the 3 groups while the leucocyte count showed an increasing trend in the blank and control groups but a decreasing trend in the experimental group.At one week post-intervention,there was a significant difference in CRP between the 3 groups (P ＜ 0.05).At 2 weeks post-intervention,the CRP level in the experimental group was significantly different from that in the blank or control group (P ＜ 0.05).At 1 to 6 weeks post-intervention,there was a significant difference in the leucocyte count between the 3 groups (P ＜ 0.05).At 6 weeks post-intervention,HE staining showed even bony staining,no obvious formation of dead bone,dense fibroplasias in the marrow cavity,and no obvious inflammatory cells in the experimental group.Conclusion NBD peptide can block inflammation in a rabbit model of tibia osteomyelitis. Key words: Peptides; Transcription factors; Osteomyelitis