Abstract
Background: Traditional chemotherapy and radiotherapy are ineffective in the treatment of renal cancer. Quercetin exhibits a broad-,spectrum anti-cancer role with only minor side effects in the treatment of many tumors, but its efficacy in renal cancer treatment is presently unknown.Methods: Renal cancer ACHN cells were cultured and treated with quecertin at various dosages. Cell viability was tested by MTT assay. Flow cytometry measured cell apoptosis. Real time PCR and Western Blot measured survivin expression, and caspase 3 activity was also detected.Results: Quecertin significantly suppressed ACHN cell proliferation and induced early cell apoptosis in a dose-dependent manner (p < 0.05). Quecertin significantly enhanced caspase 3 activity (p < 0.05), but downregulated survivin mRNA and protein expression (p < 0.05).
Highlights
Renal cancer accounts for 2 % of all adult malignancies and is the second-most common malignant tumor of the urogenital system
This suggested that quercetin dose-dependently inhibited renal cancer cell proliferation (Fig. 1)
Flow cytometry assay showed that quercetin induced significant apoptosis in ACHN cells compared to untreated cells (p < 0.05). 40 μM quercetin exhibited more obvious effect than 20
Summary
Renal cancer accounts for 2 % of all adult malignancies and is the second-most common malignant tumor of the urogenital system. Most primary renal cancers are not diagnosed until in an advanced stage as there are few typical symptoms for early diagnosis. All that remains for patients with advanced renal cancer is traditional chemotherapy and radiotherapy, yet renal cancer is non-responsive to both therapies [4]. There is still an urgent need for identifying effective treatments, along with early detection, for renal cancer remain urgently needed. Traditional chemotherapy and radiotherapy are ineffective in the treatment of renal cancer. Quercetin exhibits a broad,spectrum anti-cancer role with only minor side effects in the treatment of many tumors, but its efficacy in renal cancer treatment is presently unknown
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
