The analysis of identification for non-tuberculous Mycobacterium with molecular assay genotype Mvcobacterium kit

Abstract

Objective To identify non-tuberculous Mycobacterium（NTM） rapidly with HAIN mo- lecular assay genotype Mycobacterium kit, and investigate the advantages and disadvantages of this method. Methods Seventy-four clinical NTM isolates were collected from hospitals in Zhejiang and Anhui province. Clinical strains were identified with HAIN molecular assay genotype Mycobacterium kit. 16S rRNA gene sequencing was used to estimate and compare with this method. Results The results of kit showed that there were thirty-one M. intracellulare strains, twelve M. chelonae strains, eight M. fortuitum strains, six M. kansasii strains, five M. avium strains, three M. smegmatis strains, two M. phlei strains, two M. scrofulaceum strains and one M. gordon strain. Four strains were identified as Mycobacterium without further identification. Eight M. tuberculosis strains were identified correctly too. Compared with 16S rRNA gene sequencing, except for four strains identified as Mycobacterium, others 70 strains got the same results as 16S rRNA gene sequencing, the coincidence was 94.59%, and it could further identify thirteen Mycobacterium chelonae complex and eight Mycobacterium kansasii complex to subspecies M. abscessus and M. kansasii, respectively. If only to identify strains under the identification range of this kit, the coincidence reach to 100%, Conclusion The method of HAIN molecular assay genotype Mycobacterium kit is simple and accurate, the time is shorter and should widely be applied clinically. Key words: Non-tuberculous Mycobacterium （NTM） ; HAIN molecular assay genotype Mycobacterium kit; 16S rRNA gene sequencing