Abstract
Amylase are the most important product obtained for industrial catalyst from bacteria. The industrial dependency on enzymes and the raising demand for enzymes have increased concerns about biocatalyst. Amylase is great essential application in industries like flour industries. The objective study was to isolation, identification amylolytic bacteria and production of amylase. The isolated bacteria were characterized by morphological, physiological and biochemical tests. The highest amylase activity 5077U was achieved when the cassava concentration 1%, 30°C at pH 7 and four days incubation time without shaking. The activity of amylase was found to enhance when logarithmic phase at cassava starch as a sole carbon source. The maximum amylase enzyme activity was obtained at the logarithmic growth phase. The selected bacteria were identified based on 16S rDNA gene analysis. The 16S rRNA gene analysis revealed that amylolytic bacteria has the highest homology with Bacillus thuringiensis (99%). The selected isolate was identified as Bacillus thuringiensis after 16S rRNA gene analysis.
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