The amount of the endogenous and exogenous skeletal muscle actin mRNA in the heart of transgenic mice is affected by the genotype of the cardiac actin gene

Abstract

Abstract Both skeletal muscle and cardiac actins are co-expressed in the newborn heart. However, the amount of the skeletal muscle actin and its mRNA rapidly decreases during early development and the cardiac actin predominates in the adult heart. In BALB/c and DBA mice there is a mutation in the cardiac actin gene which is associated with decreased levels of cardiac actin mRNA and high levels of the skeletal muscle actin transcript in the adult heart. To examine the possibility that the amount of cardiac actin gene product modulates the expression of the skeletal muscle actin gene in the heart, transgenic mice carrying a tagged skeletal muscle actin gene were produced, and the expression of the endogenous and exogenous actin gene was analyzed in offspring carrying different combinations of the cardiac actin alleles. It was found that both the endogenous and exogenous skeletal muscle actin genes were expressed at low levels in the heart of adult mice homozygous for the wild-type cardiac actin gene allele, at abnormally high levels in mice homozygous for the mutated cardiac actin allele, and at intermediate levels in heterozygous mice. This shows that the level of expression of the cardiac actin gene has a trans effect on the expression of the skeletal muscle actin gene.