The Amino‐Acid Sequence of Porcine Adenylate Kinase from Skeletal Muscle

Abstract

Adenylate kinase (ATP:AMP phosphotransferase) has been purified 490‐fold from porcine muscle with a final yield of 60 mg/kg muscle. The amino‐acid composition is Asp11, Asn2, Thr14, Ser11, Glu19, Gln6, Pro6, Gly19, Ala8, Cys2, Val17, Met6, Ile9, Leu18. Tyr7, Phe5, Lys21, His2, Arg11. The protein molecule is a single polypeptide chain of 194 amino‐acid residues with an acetyl‐methionine at the N‐terminus and a lysine residue at the C‐terminus. Cyanogen bromide cleavage of carboxymethylated adenylate kinase yielded six fragments which were further degraded by using trypsin, chymotrypsin, thermolysin, subtilisin or α‐protease. Sequence data on the resulting peptides are summarized in the present report, full details are given in a supplementary paper which has been deposited at CNRS from where copies can be obtained. The primary structure of porcine adenylate kinase is: Ac‐Met‐Glu‐Glu‐Lys‐Leu‐Lys‐Lys‐Ser‐Lys‐Ile10‐Ile‐Phe‐Val‐Val‐Gly‐Gly‐Pro‐Gly ‐ Ser‐Gly20‐Lys‐Gly‐Thr‐Gln‐Cys‐Glu‐Lys‐Ile‐Val‐Gln30‐Lys‐Tyr‐Gly‐Tyr‐Thr‐His‐Leu‐Ser‐Thr‐Gly40‐Asp‐Leu‐Leu‐Arg‐Ala‐Glu‐Val‐Ser‐Ser‐Gly50‐Ser‐Ala‐Arg‐Gly‐Lys‐Met‐Leu‐Ser‐Glu‐Ile60‐Met‐Glu‐Lys‐Gly‐Gln‐Leu‐Val‐Pro‐Leu‐Glu70‐Thr‐Val‐Leu‐Asp‐Met‐Leu‐Arg‐Asp‐Ala‐Met80‐Val‐Ala‐Lys‐Val‐Asp‐Thr‐Ser‐Lys‐Gly‐Phe90‐Leu‐Ile‐Asp‐Gly‐Tyr‐Pro‐Arg‐Glu‐Val‐Lys100‐Gln‐Gly‐Glu‐Glu‐Phe‐Glu‐Arg‐Lys‐Ile‐Gly110‐Gln‐Pro‐Thr‐Leu‐Leu‐Leu‐Tyr‐Val‐Asp120‐Ala‐Gly‐Pro‐Glu‐Thr‐Met‐Thr‐Lys‐Arg‐Leu‐Leu130‐Lys‐Arg‐Gly‐Glu‐Thr‐Ser‐Gly‐Arg‐Val‐Asp140‐Asp‐Asn‐Glu‐Glu‐Thr‐Ile‐Lys‐Lys‐Arg‐Leu150‐Glu‐Thr‐Tyr‐Tyr‐Lys‐Ala‐Thr‐Glu‐Pro‐Val160‐Ile‐Ala‐Phe‐Tyr‐Glu‐Lys‐Arg‐Gly‐Ile‐Val170‐Arg‐Lys‐Val‐Asn‐Ala‐Glu‐Gly‐Ser‐Val‐Asp180‐Asp‐Val‐Phe‐Ser‐Gln‐Val‐Cys‐Thr‐His‐Leu190‐Asp‐Thr‐Leu‐Lys.