Abstract
In this study, the amelogenin-derived peptide, TVH-19, which has been confirmed to promote mineralization, was evaluated to derive its potential to induce dentinal tubule occlusion. The binding capability of fluorescein isothiocyanate (FITC)-labeled TVH-19 to the demineralized dentin surface was analyzed by confocal laser scanning microscopy (CLSM). Additionally, the sealing function of the peptide was studied through the remineralization of demineralized dentin in vitro. The adsorption results showed that TVH-19 could bind to the hydroxyapatite and demineralized dentin surfaces, especially to periodontal dentin. Scanning electron microscopy analysis further revealed that TVH-19 created mineral precipitates. The plugging rate in the TVH-19 group was higher than that in the PBS group. Moreover, energy-dispersive X-ray spectroscopy (EDX) results indicated that the calcium/phosphorus (Ca/P) ratio of the new minerals induced by TVH-19 was close to that of the hydroxyapatite. Attenuated total internal reflection-Fourier transform infrared (ATR-FTIR) spectrometry and X-ray diffraction (XRD) results indicated that the hydroxyapatite crystals formed via remineralization elongated the axial growth and closely resembled the natural dentin components. These findings indicate that TVH-19 can effectively promote dentin sealing by binding to the periodontal dentin, promoting mineral deposition, and reducing the space between the dentin tubules.
Highlights
As a global challenge in dental clinics, dentin hypersensitivity (DH) is characterized by a transient, sharp pain originating from the exposure of dentin [1]
The fluorescence on the fluorescein isothiocyanate (FITC)-TVH-19-treated demineralized sample surface was obvious, with green circles formed around the peritubular dentin (Figure 2(Ae)), while the FITC-treated sample showed the weaker fluorescence dispersion (Figure 2(Ad)) and did not gather near the peritubular dentin
In summary, the peptide TVH-19 bound to the dentin surface, forming
Summary
As a global challenge in dental clinics, dentin hypersensitivity (DH) is characterized by a transient, sharp pain originating from the exposure of dentin [1]. The prevalence of DH varies widely, depending on the mode of investigation Effective occlusion of dentin tubules to avoid exposure to stimuli from the external environment is considered to be a reliable strategy for avoiding the fluid movement and reducing dentin permeability, thereby alleviating DH and protecting the dental pulp [2,3,4]. The most important mechanisms of action of these materials include the formation of mineralized barriers and reduction of the permeability of dentin tubules. Sodium fluoride reduces dentin permeability within a short period [11], some studies have shown that the mode of action of fluoride occurs through the precipitation of calcium fluoride crystals within the tubules, which is not ideal with respect to biomimetics [12]. The existence of dental fluorosis and skeletal fluorosis makes it necessary to unravel more bioinspired non-fluoride materials as alternatives to fluoride [13,14]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
