Abstract
We have studied the cellular localization of human adenosine deaminase binding protein in human skin fibroblasts. The binding activity sediments with the membrane fractions in a subcellular fractionation, and anti-binding protein antiserum reacts with the cell surface of intact fibroblasts, as shown by indirect immunofluorescence. The surface of intact fibroblasts binds 125I-labeled adenosine deaminase with an apparent KD of 0.26 units/ml and a maximum binding capacity of 553 milliunits/mg of cell protein. This binding is inhibited by antibinding protein antiserum. We conclude that most, if not all, of the adenosine deaminase binding protein is located on the cell surface.
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