The acute toxicity of pesticide mixtures to honeybees.

Abstract

Honeybees (Apis mellifera) frequently live in complex environments where exposure to mixtures of pesticides is possible. Although several studies have expressed concern regarding the combined effects of pesticide mixtures, other studies did not find increased toxicity. Thus, the primary objective of this study was to identify peer-reviewed literature measuring the toxicity of pesticide mixtures to honeybees and determine how frequently synergistic interactions occur. Many experiments (258) were identified that met the criteria for inclusion. When considering all experiments, 34% of experiments had model deviation ratios (MDR; expected toxicity/observed toxicity) greater than 2, suggesting greater-than-additive toxicity. Twelve percent of experiments had MDR values greater than 5, with several studies exceeding 100. However, most experiments that had higher MDRs included azole fungicides or acaricides as a component of the mixture. After removal of these groups, only 8% of experiments exceeded an MDR of 2, and no experiments exceeded 5. Moreover, the influence of the azole fungicides was dose dependent. If only experiments that used azole exposure at environmentally relevant concentrations were considered, azole fungicides had limited impact on neonicotinoid insecticides. However, pyrethroid insecticides still had greater than expected toxicity with 80% of experiments having MDR values greater than 2. Acaricides also had greater than expected incidence of synergy with approximately 30% of studies reporting MDR values greater than 2. It should be noted that even the azole studies considered environmentally relevant frequently used maximum exposure rates and worst-case exposure scenarios. The primary finding is that synergy is uncommon except for a few cases where known synergists (azole fungicides) and pesticides with variable metabolism potential, such as some pyrethroids, are in combination. Future work is still needed to refine the relevance of azole fungicides at commonly occurring environmental concentrations. Integr Environ Assess Manag 2022;18:1694-1704. © 2022 SETAC.