Abstract
Acute and chronic toxicity tests were performed withDaphnia magna (Straus) and three organic solvents commonly used to facilitate the solubilization of lipophilic compounds during acute toxicity tests. The 48-hr LC50 values were: acetone 39000μl/L; dimethyl formamide, 13000μl/L; triethylene glycol, 35000μl/L. Maximum acceptable toxicant concentrations determined during the chronic toxicity tests with acetone, dimethyl formamide, and triethylene glycol were between 1400 and 2800μl/L, 1200 and 2500μl/L, and 5500 and 11000μl/L, respectively. Triethylene glycol was the least toxic solvent and is recommended as the primary choice when selecting a carrier solvent. All three solvents were sufficiently low in toxicity to suggest that the recommended usage limits (500μl/L during acute toxicity tests, 100μl/L during long-term toxicity tests) are adequate for the prevention of solvent related toxicity toD. magna.
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