Abstract
Microsomal suspensions were prepared in parallel from normal and regenerating rat livers, and their activity for amino acid incorporation was determined in incorporation systems containing C 14- l-leucine as the labeled component and standard amounts of normal cell fluid as the source of activating enzymes. The concentrations of the microsomal suspensions were determined by analyses of total nitrogen, protein, ribonucleic acid (RNA) and glucose-6-phosphatase. When the relative microsomal activities for amino acid incorporation were compared at different periods after hepatectomy a rapid rise was observed after a lag period of about 12–14 hours. This rise occurred simultaneously with a change in the composition of the microsomal material. On the basis of equal protein concentrations the amounts of RNA increased while the activity of glucose-6-phosphatase decreased. The change may indicate modified proportions between the granular and lamellar constituents of the endoplasmic reticulum, from which the microsomal material emanates. The increased microsomal activities during the regeneration period may be conditioned by a higher average concentration of nucleo-protein granules connected with the reticular membranes.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
