Abstract
Ekor naga’s leaf (Rhaphidophora pinnata (Lf) Schott) is a type of vines and climbing plant. The leaves are elongated round and hollowed inside. This plant had been using for the treatment of breast cancer. Extraction with percolation method has been done in ekor naga’s leaves with ethanol, and fractionated by nhexane, chloroform and ethyl acetate using liquid-liquid extraction (LLE). Cytotoxicity assay of ethanol extract, n-hexane fraction, chloroform fraction, ethyl acetate fraction and water fraction against MCF-7 cells were done using MTT method (3-(4,5-dimetiltiazol-2-il)-2,5-diphenyl tetrazolium bromide). Phytochemical screening results showed the presence of the compounds such as triterpenoida/steroid, alkaloid, flavonoid, tannin, and saponin. n-hexane fraction was positive for the presence of triterpenoida/steroid, chloroform fraction containing alkaloids, saponin and triterpenoid; ethyl acetate fraction contained, flavonoid, tannin, and the fraction of water indicated the presence of tannin and saponin. Secondary metabolite compounds in ethanol extract, chloroform fraction and ethyl acetate fraction gave positive results against MCF-7 cells. Cytotoxicity assay of MCF-7 cell line showed that crude ethanol extracts had 112.240 mcg/ml IC50 chloroform fraction IC50 was 59.082 mcg/ml, and ethyl acetate fraction IC50 was 812.663 mcg/ml.
Highlights
Breast cancer is one of the worldwide deathly cancer type after lung, liver and colon cancer
Cytotoxicity assay of ethanol extract, n-hexane fraction, chloroform fraction, ethyl acetate fraction and water fraction against Michigan Cancer Foundation-7 (MCF-7) cells were done using MTT method (3-(4,5-dimetiltiazol-2-il)-2,5-diphenyl tetrazolium bromide)
Phytochemical screening results showed the presence of the compounds such as triterpenoida/steroid, alkaloid, flavonoid, tannin, and saponin. n-hexane fraction was positive for the presence of triterpenoida/steroid, chloroform fraction containing alkaloids, saponin and triterpenoid; ethyl acetate fraction contained, flavonoid, tannin, and the fraction of water indicated the presence of tannin and saponin
Summary
Breast cancer is one of the worldwide deathly cancer type after lung, liver and colon cancer. Some research showed that many of the plants contain a variety of chemical compounds as potential anticancer. One of those things that has became scientists’ observation is traditional medicine. MCF-7 cells have been used for a variety of in vitro research on breast cancer because they have some of the same characteristics associated with breast epithetlial estrogen in the form of the ability to process estradiol via estrogen receptors in the cytoplasm. In order to find new drugs for the treatment of breast cancer, the in vitro cytotoxicity test of the ethanol extract (crude) and fractions (n-hexane, chloroform, ethyl acetate and water) of ekor naga leaves against MCF-7 cells had been done
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
