Evaluation of Viburnum grandiflorum for its in-vitro pharmacological screening

Abstract

In the present study the crude ethanolic and its succeeding solvent fractions of the aerial and roots parts of Viburnum grandiflorum were investigated for their in-vitro pharmacological (antifungal, phytotoxic and insecticidal) profile. In case of antifungal activity the n-hexane fraction was active against Microsporum canis having 35% zone of inhibition and ethyl acetate fraction were active against Fusarium solani with 10% zone of inhibition. The ethyl acetate fraction of aerial part was the most significant antifungal against F. solani with 30% zone of inhibition while the crude extract was 10% active against M. cani. The n-hexane, chloroform fractions and ethanolic extract of the root were significant phytotoxic having 70, 75 and 80% inhibition respectively. In case of aerial part the most significant phytotoxic effect was revealed by ethyl acetate with 85% inhibition followed by ethanolic extract with 80% growth of inhibition. The crude ethanolic extract of the aerial part showed 20, 40 and 40% mortality against Tribolium castaneum, Callosbruchus analis and Rhyzopertha dominica respectively.The n-hexane, chloroform and ethyl acetate fractions showed 20% mortality against C. analisand R. dominica. All the tested samples were significant insecticidal against R. dominica. The n-hexane fraction of root was failed to produce any insecticidal effect, while the chloroform fraction was effective against C. analis and R. dominica with 20% mortality. The crude ethanolic extract and its ethyl acetate fraction showed 40% mortality C. analis and R. dominica. Key word: Viburnum grandiflorum, antifungal, phytotoxic and insecticidal.