Abstract
Background: The objective of the present study was to evaluate the acid tolerance response and pH adaptation when Enterococcusfaecalis interacted with extract of lime ( Citrus aurantiifolia). Methods: We used E. faecalis ATCC 29212 and lime extract from Aceh, Indonesia. Both materials were analyzed for their pH adaptation, acid tolerance response, adhesion assay, and mass profiles using a light microscope with a magnification of x1000. Further, statistical tests were performed to analyze both correlation and significance of the acid tolerance and pH adaptation also the interaction activity. Results:E. faecalis was able to adapt to a very acidic environment (pH 2.9), which was characterized by an increase in its pH (reaching 4.2) at all concentrations of the lime extract (p < 0.05). E. faecalis was also able to provide acid tolerance response to lime extract based on spectrophotometric data (595 nm) (p < 0.05). Also, the interaction activity of E. faecalis and the lime extract was relatively stable within 6 up to 12 hours (p < 0.05), but it became unstable within 24-72 hours (p > 0.05) based on the mass profiles of its interaction activity. Conclusions:E. faecalis can adapt to acidic environments (pH 2.9-4.2); it is also able to tolerate acid generated by Citrus aurantiifolia extract, revealing a stable interaction in the first 6-12 hours.
Highlights
Enterococcus faecalis is a significant agent in the pathogenesis of root canal infections, especially in post-endodontic treatment, with a prevalence of 24–77% in these infections[1]
The analysis of variance (ANOVA) test showed that lime extracts and exposed time gave the significant effect on the pH, optical density of acid tolerance respond of E. faecalis in lime extract and optical density of adhesion of interaction activity between E. faecalis and lime extract on biofilm (p
The interaction between lime extract and exposed time gave the significant effect on the pH, optical density of acid tolerance respond of E. faecalis in lime extract and optical density of adhesion of interaction activity between E. faecalis and lime extract on biofilm (p
Summary
Enterococcus faecalis is a significant agent in the pathogenesis of root canal infections, especially in post-endodontic treatment, with a prevalence of 24–77% in these infections[1]. It can adapt to acidic conditions, including living in the dentin tubule of a closed root canal with a smear layer It can express the dominant biofilm protein to maintain its attachment to host cells[2]. The objective of the present study was to evaluate the acid tolerance response and pH adaptation when Enterococcus faecalis interacted with extract of lime (Citrus aurantiifolia). Methods: We used E. faecalis ATCC 29212 and lime extract from Aceh, Indonesia Both materials were analyzed for their pH adaptation, acid tolerance response, adhesion assay, and mass profiles using a light microscope with a magnification of x1000. Conclusions: E. faecalis can adapt to acidic environments (pH 2.9–4.2); it is able to tolerate acid generated by Citrus aurantiifolia extract, revealing a stable interaction in the first 6–12 hours
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