THE ABILITY OF PHOTOCHEMICAL DECOMPOSITION PRODUCTS OF THE ROUNDUP TO INDUCE OXIDATIVE STRESS IN BACTERIAL CELLS

Abstract

BACKGROUND: The most common herbicide in agriculture for weed control is Roundup (Glyphosate), the active ingredient of which is N-phosphonomethylglycine (N-FMG). It is necessary to assess the hazard of the products of photochemical decomposition of N-FMG formed under the influence of solar UV and ozone.
 AIM: Using E. Coli lux-biosensors, a study of the ability of N-FMG photochemical degradation products to induce oxidative stress in bacterial cells.
 MATERIALS AND METHODS: Photochemical oxidation of an aqueous solution of N-FMG, mass spectrometry for the determination of N-FMG photochemical oxidation products and determination of their activity using E.coli (pSoxS-lux) and E.coli (pKatG-lux).
 RESULTS: Using biosensors, it was shown that the products of photochemical decomposition of N-PMG cause an increase in the concentration of superoxide anion radical and H2O2 in E. coli cells, which induces oxidative stress in the bacterial cell.
 CONCLUSION: The photochemical decomposition product of N-PMG induces the formation of superoxide anion radical and H2O2 in bacterial cells.
 
 Keywords: glyphosate (roundup, N-phosphonomethylglycine), photochemical degradation, mass spectrometry, E. coli, oxidative stress, biosensors pSoxS-lux, pKatG-lux.