The ability of photochemical decomposition products of the Roundup to induce oxidative stress in bacterial cells

Abstract

BACKGROUND: A common non-selective systemic herbicide Roundup (Glyphosate, active ingredient N-phosphonomethylglycine, N-PMG) is used to control perennial weeds. It is necessary to assess the hazard of the products of photochemical decomposition of N-FMG formed under the influence of solar UV and ozone. AIM: Using lux-biosensors based on Escherichia coli, studying the ability of N-FMG photochemical degradation products to induce oxidative stress in bacterial cells. MATERIALS AND METHODS: The work used the active substance of the herbicide Roundup N-phosphonomethylglycine (N-PMG), biosensors E. coli (pSoxS-lux), E. coli (pKatG-lux). UV radiation, Mass spectrometry. RESULTS: Using biosensors, it was shown that the products of photochemical decomposition of N-PMG (2-(N-hydroxymethyl-hydroxyamine) ethanoic acid) cause an increase in the concentration of superoxide anion radical and H2O2 in E. coli cells, which induces oxidative stress in the bacterial cell. CONCLUSIONS: The photochemical decomposition product of N-PMG (2-(N-hydroxymethyl-hydroxyamine) ethanoic acid) induces the formation of superoxide anion radical and H2O2 in bacterial cells.