Abstract
Anthurium andraeanum Lind. is a popular potted and cut-flower plant with an attractive spathe and foliage. It is native to tropical rainforest areas and is able to blossom throughout the year under suitable conditions. However, various abiotic stresses seriously restrict the ornamental value of A. andraeanum and increase the costs of cultivation. A dark green (dg) leaf color mutant of A. andraeanum ‘Sonate’, which accumulates high levels of anthocyanin, has shown increased vigor and tolerance to stresses during cultivation and is, thus, an ideal germplasm for studying stress tolerance in this species. Here, we show that the anthocyanin content in dg mutant plants at different stages of leaf development was higher than in wild-type (WT) plants, and the ability to tolerate under low-temperature (LT, 14 °C) stress was stronger in dg than in WT plants. RNA-Seq of cDNA libraries from young leaves of dg and WT identified AabHLH35 as a differentially expressed gene (DEG) that was significantly up-regulated in dg. Furthermore, heterologous expression of AabHLH35 improved tolerance to cold and drought stresses in Arabidopsis. These results have built an important molecular foundation for further study of stress tolerance in A. andraeanum.
Highlights
Anthurium andraeanum Lind. is a popular potted and cut-flower plant with an attractive spathe and foliage
We show that the anthocyanin content in dg mutant plants at different stages of leaf development was higher than in wild-type (WT) plants, and the ability to tolerate under low-temperature (LT, 14 ◦ C) stress was stronger in dg than in WT plants
A. andraeanum plants with consistent growth under a 12-h photoperiod (180 μmol m−2 s−1 light intensity) at 25 ◦ C were used for cold stress treatment (14 ◦ C for 12 days and recovery at 25 ◦ C for 4 days)
Summary
Anthurium andraeanum Lind. is a popular potted and cut-flower plant with an attractive spathe and foliage. Is a popular potted and cut-flower plant with an attractive spathe and foliage. It is native to tropical rainforest areas and is able to blossom throughout the year under suitable conditions, but abiotic stresses during cultivation, especially cold stress, are serious threats to its growth and development and compromise the economic and ornamental importance of this species. We acquired a spontaneous leaf color mutant, dark green (dg), which originated from a leaf color chimera population of tissue culture-derived plantlets in A. andraeanum ‘Sonate’ [1]. Dg showed stronger growth vigor and more stress tolerance than wild-type (WT) plants (data not shown). The chilling injury traits of WT plants were more obvious than those of dg at low temperature environment (Figure S1). The molecular mechanism in dg that improves plant tolerance to stresses remains elusive
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