Abstract
BackgroundBiofilm is one of the causes of antibiotic resistance. One of the biofilm-producing bacteria is Staphylococcus epidermidis which has been proven to infect long-term users of urinary catheters and implant devices. The 1-monolaurin compound has been known to have an antimicrobial effect. However, its effect on clinical isolates of S. epidermidis in producing biofilm has not been established. This study was conducted to investigate the effect of 1-monolaurin towards biofilm forming clinical isolates of S. epidermidis.MethodsThe experiment used micro broth dilution technique which consists of test group (1-monolaurin), positive control group (rifampicin), solvent group, negative control group (clinical isolate of S. epidermidis), and media group (TSB media). The Minimal Inhibition Concentration (MIC) was determined by incubating bacteria added with 1-monolaurin (1000–1953 μg/mL) or rifampicin (250–0,488 μg/mL) for 24 h. The MIC was determined visually. After that, the incubated bacteria was cultured in TSA media to determine Minimal Bactericidal Concentration (MBC). The assessment of Biofilm inhibitory Concentration (BIC) and Biofilm Eradication Concentration (BEC) was conducted with the same way, the difference was BIC intervened directly with compound meanwhile BEC was incubated for 24 h in 37 °C before the intervention. Then, the specimen was reincubated to grow biofilm at the microplate, washed with PBS and stained with 1% of crystal violet. The optical density (OD) was measured at a wavelength of 595 nm. The percentage of BIC and BEC then were calculated, continued to probit analysis regression to determine the BIC50, BIC80, BEC50, and BEC80.ResultsThe MIC dan MBC of 1-monolaurin and rifampicin were > 1000 μg/mL, > 1000 μg/mL, ≤0.488 μg/mL, and 1.953 μg/mL respectively. BIC50 and BIC80 of 1-monolaurin and rifampicin were 26.669 μg/mL, 168.688 μg/mL, 0.079 μg/mL, and 0.974 μg/mL respectively. The BEC50 and BEC80 of 1-monolaurin and rifampicin were 322.504 μg/mL, 1338.681 μg/mL, 5.547 μg/mL, dan 17.910 μg/mL respectively.ConclusionThe 1-monolaurin can inhibit growth and eradicate the biofilm formed by clinical isolates of S. epidermidis, however, it has neither inhibit nor kill planktonic cells of S. epidermidis.
Highlights
Biofilm is one of the causes of antibiotic resistance
Preparing clinical isolate of Staphylococcus epidermidis The clinical isolate of bacteria producing biofilm S. epidermidis was prepared in suspension by mixing the pellets of S. epidermidis clinical isolates with 0.9% sterile NaCl
According to the Clinical & laboratory standards institute (CLSI) [12], the S. epidermidis clinical isolate which used in this study showed its sensitivity to antibiotics
Summary
Biofilm is one of the causes of antibiotic resistance. One of the biofilm-producing bacteria is Staphylococcus epidermidis which has been proven to infect long-term users of urinary catheters and implant devices. Its effect on clinical isolates of S. epidermidis in producing biofilm has not been established. The appearance of microorganisms growth that often occurs is biofilm formation. Microorganism produces Extracellular Polymeric Substance (EPS) that facilitate attachment and biofilm formation as a result change host phenotype. The first stage includes an initial attachment that can occur actively or passively This process depends on the physicochemical components of bacteria and their surface components. The third stage has entered the initial process of establishing an architecture of biofilm (microcolony formation). Microcolony formation resulted from the accumulation and growth of microorganisms and the production of EPS. This strengthens the bacterial bond with the host. Bacterial cells will return to their planktonic cells and come out of the biofilm to form new colonies [2]
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