Abstract
Unused feed containing protein in the water affects the fish survivability under the recirculating aquaculture systems. Microbial communities play important roles in nutrient cycling in the aquatic ecosystems, however, bacteria that may help in protein degradation remains underexplored. This study aimed to identify the proteolytic bacteria obtained from aquaculture system based on 16S rRNA genes. Bacteria were isolated using non-selective medium and then assayed for proteolytic activity on skim milk agar. Characterizations were conducted for selected proteolytic bacteria before subjected to Sanger dideoxy DNA sequencing. The results of BLAST show that five representative isolates are closely related to Flavobacterium nitratireducens, Micrococcus aloeverae, Acinetobacter baumannii, and Exiguobacteríum indicum at the level similarity of 99%. The nucleotides of collected proteolytic bacterial strains have been deposited in NCBI Genbank. Finding of those proteolytic bacteria in the recirculating aquaculture system may lead the further ecological studies about their roles in the ecosystem.
Published Version
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