Thalamic distribution of projection neurons to the primary motor cortex relative to afferent terminal fields from the globus pallidus in the macaque monkey.

Abstract

To examine quantitatively the pathway from the internal segment of the globus pallidus to the primary motor cortex through the thalamus, we compared the distribution of thalamocortical neurons projecting to the motor cortex with the distribution of afferent terminal fields from the pallidum in the ventrolateral nuclear group of the thalamus in four Japanese monkeys by using the anterograde and retrograde double-labeling method. In each monkey, different fluorescent retrograde tracers (Fast Blue and Diamidino Yellow) were injected separately into the distal and proximal forelimb areas of the primary motor cortex after physiological mapping with intracortical microstimulation. In the same individual monkeys, an anterograde tracer, wheatgerm agglutinin conjugated to horseradish peroxidase, was injected into the internal segment of the globus pallidus after the forelimb part was identified physiologically. A small group of projection neurons to the distal and proximal representations of the motor cortex were found in the terminal fields from the pallidum, but a majority of the projection neurons were distributed outside the terminal area in the thalamus. These results confirm the existence of the pathway from the pallidum through the thalamus to the primary motor cortex, but also indicate that the primary motor cortex receives its major thalamic inputs from outside of the pallidal projection area, and that the pallidum sends its major outputs to nonprimary motor areas through the thalamus.