Abstract
Lotus corniculatusL. is a tetraploid (2n= 4x= 24) forage legume which has had limited inheritance studies. The purpose of this research was to develop a genetic map and characterize the genome ofL. corniculatususing molecular markers. A linkage map was constructed forL. corniculatususing information from 217 markers mapped as single‐dose restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), inter‐simple sequence repeat (ISSR), sequence tagged site (STS), and isozyme markers and five duplex RFLP markers. Formulas for calculating maximum likelihood estimates of recombination involving codominantly scored duplex loci in tetrasomic species from direct count data are made available. Six composite linkage groups were constructed from the combined data of four homologous linkage groups anchored by shared RFLP loci. Three of the composite groups (Groups 2, 3, and 4) were well defined, two groups (Groups 5 and 6) were poorly discriminated because of apparent marker duplication found between them, and one group (Group 1) was poorly defined because of an apparently high degree of differentiation between its homologous members. This first genetic map forL. corniculatusspans 572.1 centimorgans (cM) and serves to provide a foundation for the genetic analysis of complex traits and marker‐aided selection.
Published Version
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