Tetrahydro-2-furanmethanol pretreatment of eucalyptus to enhance cellulose enzymatic hydrolysis and to produce high-quality lignin

Abstract

In this study, an organosolv process based on tetrahydro-2-furanmethanol/acid aqueous solution was developed to release lignin and hemicelluloses from eucalyptus for the enhancement of enzymatic hydrolysis. The pretreatment with 80% THFA aqueous solution containing 1% HCl at a solid to liquid ratio of 1:10 was conducted at 120–180 °C for 30 min. Under the serious conditions, the cellulose-rich residue had a high proportion of cellulose up to 87.31%. The enzymatic hydrolysis rate of the cellulose-rich fraction was 94.18%, which was enhanced by 7.3-fold as compared to that of the raw material without pretreatment. The fractionation process resulted in a high delignification rate of 93.95%. The lignin produced with medium molecular weight (2190 g/mol) and high purity (contaminated sugar content 0.6%) was a good substrate for further application as chemicals.